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length brca1 gene  (OriGene)


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    Structured Review

    OriGene length brca1 gene
    <t>BRCA1</t> facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.
    Length Brca1 Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+brca1+gene/pmc04413257-132-10-15?v=OriGene
    Average 96 stars, based on 738 article reviews
    length brca1 gene - by Bioz Stars, 2026-07
    96/100 stars

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    1) Product Images from "Identification of Novel Breast Cancer Subtype-Specific Biomarkers by Integrating Genomics Analysis of DNA Copy Number Aberrations and miRNA-mRNA Dual Expression Profiling"

    Article Title: Identification of Novel Breast Cancer Subtype-Specific Biomarkers by Integrating Genomics Analysis of DNA Copy Number Aberrations and miRNA-mRNA Dual Expression Profiling

    Journal: BioMed Research International

    doi: 10.1155/2015/746970

    BRCA1 facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.
    Figure Legend Snippet: BRCA1 facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.

    Techniques Used: Over Expression, Expressing, Transfection, Plasmid Preparation, Western Blot, Quantitative RT-PCR, In Vivo, Luciferase, Standard Deviation



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    length brca1 gene  (OriGene)
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    OriGene length brca1 gene
    <t>BRCA1</t> facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.
    Length Brca1 Gene, supplied by OriGene, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+brca1+gene/pmc04413257-132-10-15?v=OriGene
    Average 96 stars, based on 1 article reviews
    length brca1 gene - by Bioz Stars, 2026-07
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    commercial full-length gene sequencing test for brca1 and brca2  (Myriad Genetics)
    90
    Myriad Genetics commercial full-length gene sequencing test for brca1 and brca2
    <t>BRCA1</t> facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.
    Commercial Full Length Gene Sequencing Test For Brca1 And Brca2, supplied by Myriad Genetics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/length+brca1+gene/pmc06096880-99-20-2?v=Myriad+Genetics
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    commercial full-length gene sequencing test for brca1 and brca2 - by Bioz Stars, 2026-07
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    Image Search Results


    BRCA1 facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.

    Journal: BioMed Research International

    Article Title: Identification of Novel Breast Cancer Subtype-Specific Biomarkers by Integrating Genomics Analysis of DNA Copy Number Aberrations and miRNA-mRNA Dual Expression Profiling

    doi: 10.1155/2015/746970

    Figure Lengend Snippet: BRCA1 facilitates miR-143 and miR-145 processing. In order to confirm whether BRCA1 facilitates miR-143 and miR-145 processing, BRCA1 overexpression and siBRCA1 expression plasmids were transfected into MCF-7 cells. The empty vector or scramble siRNA expression vector served as controls. (a) BRCA1 mRNA level and (b) protein level were examined by qPCR and Western blot, respectively, in stable transfected cells, normalized by beta-actin ( ∗ P < 0.05 as compared with mock control; n = 3). Then, the expression levels of the primary (pri), precursor (pre), and mature (mat) forms of the indicated miRNAs were examined in human BRCA1 (c) overexpressed and (d) knock-down MCF-7 cells using qRT-PCR analysis. Pri- and pre-miRNAs were normalized by beta-actin, and mature miRNA was normalized by U6 snRNA ( ∗ P < 0.05 as compared with mock control; n = 3). Meanwhile, in vivo monitoring assay of pri-miRNA processing in (e) BRCA1 overexpression or (f) knock-down MCF-7 cells carrying miR-143 or miR-145 at the 3′ untranslated region of the luciferase gene. The intensities were normalized by Renilla luciferase and are shown as fold induction as compared with an empty pmirGLO vector ( ∗ P < 0.05; n = 3). Error bars represent standard deviation.

    Article Snippet: MCF-7 cells were transfected with a pCMV6-XL4 vector containing the full-length BRCA1 gene purchased from Origene (Beijing, China) (empty vector as control) or pSilencer siRNA expression vector (Ambion) containing BRCA1 specific shRNA expression cassette (scramble shRNA expression vector as siRNA control).

    Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Western Blot, Quantitative RT-PCR, In Vivo, Luciferase, Standard Deviation